Not a big deal for oxy but the acetylene shouldn't be used right away if it was transported laying down.The acetone which usually resides in the bottom of the cyl gets stirred up and can be sent out through the regulator if not given time to settle to the bottom again.
Which on rare occasions makes a nice flare!
Not that I would know about this, of course...
I always transport cylinders on their side, end cap facing the BACK of the vehicle should I need to stop. Generally speaking, I just throw in a couple blocks of wood to keep them from rolling. None of the bulk cylinder transport racks I've seen offer a method to positively restrain the bottle from leaving the rack. They just rattle around in there, a couple ratchet straps keeping them in a group against one or two walls of the rack.
From my reading of pressure bottle certification tests and studies, I figure if the hit is hard enough to break off the valve cap, it's probably enough to rupture the bottle or upset the friction weld at the bottle cap. With that kind of a force, nothing short of a positive retention fixture in the middle of the truck bed would keep them restrained in that kind of a collision.
Edit:
What worries me MUCH more are older cylinders. In some cases, they have been proven to be brittle and failure of the bottle wall results in a LOT of shrapnel. A high pressure bottle should be relatively ductile, to prevent the generation of shrapnel if it fails. I'll see if I can dig up the report.