The single span has maximum bending moment in the middle, and only shear at the supports. The multi-span case also has a bending moment at the center support, so it is less obvious where it would break. In simple terms, if you stand where it says "Load" on the left, and a big guy jumps up and down where it says "Load" on the right - you will experience some up/down motion as well, as the beam snakes like the letter "S".
A good design considers both deflection and strength. I once built a small loft using 2x6 joists and while it never fell down, walking on it was a bit unnerving.
I used this online tool a month ago, to determine beam sizing for a long span loft in my barn. They offer free access for a short period of time. I'm not suggesting that you run it yourself (OK if you want to mess around), but perhaps some other ME or CivE on here would be willing to check your proposal out. My free subscription has expired, and I don't really want to pay for more time, not to mention I have other projects going on. You have to know dimensions, loading, and be able to sketch the cross section of the Cee purlins online. Then the program will give you both stress and deflection.
https://skyciv.com/structural-software/beam-analysis-software/
It's easy to say that maybe you want the deck to be 3 or 4 times stronger than the biggest party you'd ever throw, with the heaviest people you know standing around, but I'm not sure what a good standard is for deflection. All I can say is that you don't want the floor moving very much under your feet. Perhaps a CivE can chime in here if there is a standard used in building construction.
All that said - I have a feeling (not a blessing, only a feeling !) that after you do your due diligence and check the design - it's going to be fine. Not too many people make decks out of steel I-beams !